Bioneer EcoQprep™ mRNA Kit with 3 mL Magnetic Nanobead-Oligo dT (10 mg/mL)

Bioneer EcoQprep™ mRNA Kit with 3 mL Magnetic Nanobead-Oligo dT (10 mg/mL) EcoQprep™ mRNA Kit is a magnetic beadbased system for isolating high-quality mRNA with poly(A) tails from cultured cells, animal tissues, and plant tissues. Using oligo(dT) magnetic nanobeads and the EcoQprep™ Magnetic Separation Rack, the kit provides a faster and simpler workflow than traditional spin column methods. Because it does not require centrifugation, it reduces energy use and plastic waste. The purified mRNA is ready for downstream applications such as RT-PCR, RT-qPCR, cDNA synthesis, and gene cloning. Features: High-purity, high-yield mRNA isolation Oligo(dT) magnetic nanobeads selectively capture polyadenylated mRNA, efficiently removing genomic DNA and ribosomal RNA to deliver highly pure results. Eco-friendly nucleic acid extraction Compared with conventional column filter methods, the magnetic bead–based workflow reduces plastic waste. Broad sample compatibility Enables high-quality mRNA extraction from diverse sources, including mammalian cells, animal tissues, and plant tissues. Rapid processing time Magnetic bead technology allows mRNA extraction in approximately 10 minutes* Minimal equipment required Only the EcoQprep™ Magnetic Separation Rack(TM-1012) is needed—no centrifuge or additional instruments are required. Automation compatible Fully compatible with the ExiPrep™ 96 Lite (A-5250, RUO) automated nucleic acid extraction system, enabling simultaneous processing of up to 96 samples. (*excluding sample pretreatment) Applications: cDNA synthesis Reverse transcription PCR(RT-PCR) Reverse transcription quantitative PCR(RT-qPCR) Northern blot analysis RNA sequencing Table-1 Figure 1: Total RNA was purified using the AccuPrep® Universal RNA Extraction Kit, and polyadenylated mRNA was purified using the EcoQprep™ mRNA Kit. RNA yield (µg) and purity (A260/A280) were measured with a spectrophotometer. The mRNA ratio in total RNA (%) was calculated from the proportion of mRNA yield relative to total RNA yield. Samples tested included HeLa cells (2 10 cells), mouse liver (10 mg), mouse brain (10 mg), and broccoli (40 mg). Figure 2. Comparison of real-time two-step RT-qPCR amplification of HPRT1 from HeLa cells using total RNA and mRNA purification kits. HeLa cells (2 10 2 10 cells) were used as input samples. Total RNA was purified with the AccuPrep® Universal RNA Extraction Kit, and polyadenylated mRNA was purified with the EcoQprep™ mRNA Kit. Amplification of the HPRT1 gene was performed by two-step RT-qPCR. [Total RNA] Amplification curves obtained from total RNA. [mRNA] Amplification curves obtained from mRNA. [Table] Ct values of HPRT1 detected from serial dilutions of HeLa cells. UD = undetermined. Literature/Support Brochure EcoQprep™ Series (EN) EcoQprep™ mRNA Kit Flyer Manual User Guide - EcoQprep™ mRNA Kit(EN) One Page Protocol - EcoQprep™ mRNA Kit MSDS MSDS - EcoQprep™ mRNA Kit MRD Buffer EN.pdf MSDS - EcoQprep™ mRNA Kit MRW1 Buffer EN.pdf MSDS - EcoQprep™ mRNA Kit MRW2 Buffer EN.pdf MSDS - EcoQprep™ mRNA Kit MRW3 Buffer EN.pdf MSDS - EcoQprep™ mRNA EM Buffer EN.pdf